FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285935
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC531074.129835489352105No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC342432.6628873154552912No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC296252.3037711859464123No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC194291.5108850758397585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64430.5010362110059995No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA41380.32178920396443056No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA32340.25149016085571974No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA22460.17465890577673054No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG22310.17349243935346653No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA22120.17201491521733211No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG21010.1633830636851785No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT15640.12162356573232706No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT15560.12100145030658625No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAA14850.11548017590313663No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG14540.11306947862839102No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG14240.110736545781863No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG13280.10327116067297337No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA13050.10148257882396855No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC13050.10148257882396855No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGA12920.10047164125713975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG95400.0130.804661
GTCTCTC124400.0124.025342
AGCTGGT127200.0121.634849
TCAGCTG127550.0121.4704067
TCTCTCA130550.0118.6704943
AGTCTCT131750.0117.243291
CTCAGCT132800.0116.776736
CAGCTGG135700.0114.2811368
CTCTCAG136850.0113.4572144
TCTCAGC144150.0107.532325
CTCAAAC155950.081.437029
GGCTCAA156250.081.1885157
GCTCAAA157850.080.411188
GATGGCT159950.079.008714
TGGCTCA164150.077.0618746
CTGATGG164300.076.644492
ATGGCTC170500.074.310965
TGATGGC188600.066.969283
TCAAACA161950.052.191039
AGTCTAT803.8231254E-545.1344221
Produced by FastQC (version 0.11.5)