FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353518
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA3900.0118.254921
TACGGAT5150.0111.9403461
TTCGAAG2850.0111.252991
CCTTACG4200.0109.808141
TGACTAG4500.0108.893081
CGATTGA3850.0108.5603261
TAGTTGG5350.0106.408711
TGACGGC3400.0105.972931
CTTTGCG3000.0105.690341
GGATTAC5800.0105.607511
TTGAACG4550.0104.528911
ACGCCGA4900.0104.415781
GGTATTG4050.0103.199321
AGCGAAC2450.0102.827249
CAATGGA6550.0102.1992958
GGAATAA6450.0100.551071
GTAAACG2600.099.663338
AGGCGTA2250.099.1711658
TTACGGA1600.098.971229
AATCCGA2850.098.497689
Produced by FastQC (version 0.11.5)