FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722630
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTG2750.0133.54441
AGTATTG750.0124.7987448
CCTTACG2550.0121.427351
CGATTGA2050.0119.4299551
GTACGAA1250.0115.198848
TACCTTA2400.0108.0138551
TCGTTAC1800.0107.998918
TGTTCGA950.0106.1041959
TAGTTGG1700.0105.8959351
CATCGAA4000.0104.413391
ACGAGCC2150.0103.827271
CCGGGAT2400.0102.0130841
CCGTTCA2200.0101.467571
CACTCGT502.055458E-10100.8059543
GTTATGC1500.0100.798998
TCCGACG1800.099.9989859
GCGTTAA1300.099.6982046
AATACGC1300.099.69139
GTATTGT1450.099.3162162
TACGGGC3150.098.298331
Produced by FastQC (version 0.11.5)