FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706513
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGA1000.0122.40622
TAATTCG301.1479278E-7119.997584
TACCTTA2750.0117.899251
CGATTGA1600.0117.0805051
TACGGAT1600.0112.577411
CCTTACG2450.0111.7503051
CTTTGCG3100.0111.560581
ACTTCGA1050.0109.712079
GGTATTG2650.0108.754021
AGCGAAC1350.0106.664529
TGACTAG1500.0105.672661
GCGCGAT1300.0105.303171
ACGCCGA2950.0105.021361
GCAGTTA3050.0103.940321
GTACGAA1600.0103.497918
CAACGCA700.0102.8550648
TGTTCGA1350.0101.33139
CTAATGG2850.0101.122161
TTTATGC2650.0100.5974661
CATCGAA2950.0100.136641
Produced by FastQC (version 0.11.5)