FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721066
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCA1700.0122.8115163
GGTATTG3200.0121.496541
CCTTACG2400.0119.99661
CGATTGA1750.0119.31091
TTTGCGA550.0117.8066562
TGTTCGA1100.0117.798499
GGTCGAA950.0113.665227
CTCGAAA2250.0111.996821
TAGTTGG1950.0110.766081
CTTTGCG2100.0109.711161
GTTATGC1050.0109.695958
GTCGTAA1950.0107.073881
TTACGCG1350.0106.648849
TGTAATG950.0106.087549
TTAGCGA750.0105.589676
GCGCGAT1450.0104.27291
TGACGGC1750.0102.8542251
TCGACCA1050.0102.839968
CCGTTCA3650.0102.5724261
TAAACCG2000.0100.797141
Produced by FastQC (version 0.11.5)