FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891392
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA2300.0118.952128
CGATTGA2150.0117.283911
TAAACCG2400.0117.074471
GGTATTG3300.0115.709981
TACCTAT3300.0113.5267641
CCTTACG2950.0112.342651
TGTTCGA1450.0109.2373359
CTAATGG3850.0108.536581
ACTTTAG1600.0107.9963
TACGAAA1900.0106.101349
ACGAGCC2650.0106.0297241
TACGGAT1650.0104.793941
CGTGCGT1250.0103.676173
TAGTTGG2300.0103.370111
TACCTTA3350.0103.229851
GTCGTAA2400.0102.0649261
TGTAATG1200.0101.996229
TAGGCGT1000.0100.7962653
CTTTGCG2950.0100.131491
AGTTGTA1300.099.6886148
Produced by FastQC (version 0.11.5)