FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869558
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG1650.0122.193531
GGTATTG3800.0121.274781
TAGTTGG1650.0117.8294751
CTAATGG3450.0110.61931
TATTAGC1250.0109.43799
TACCTTA3500.0109.0390241
GTACGAA1850.0108.970898
TTGGCAA3000.0108.010351
TACGGAT2200.0108.010351
TATTACC3150.0107.4388661
CACGGTA2650.0105.960238
GCCTCTA2050.0105.375951
TTACGCG1650.0104.725269
TATTGAG1600.0103.503973
GTCGTAA2550.0101.65681
TAAACCG2200.0101.464271
GTATTAG1350.0101.331398
CTTTGCG3200.0101.259711
CGATTGA2350.0101.116071
AGTATTG1000.0100.798078
Produced by FastQC (version 0.11.5)