FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944607
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTG4300.0123.986891
TTTAGAC550.0117.881685
CGATTGA2350.0116.500621
ACGCCGA3650.0112.510881
GTTATGC1950.0107.04398
GGAATAA3950.0105.789711
CTTTACG1500.0105.567449
CCTTACG3150.0105.210681
TTGGACG1800.0104.061572
GTATAGT1250.0103.648038
CTAATGG3000.0103.266561
TGACTAG2100.0102.9234851
TAGTTGG2600.0102.5276261
TTCGAAG2600.0102.5276261
TTGAACG1850.0101.254461
TTTGCGA1000.0100.859672
TGCGTGG3650.0100.667631
TACGAAA1650.0100.332699
ACCGTAA3450.0100.2385251
ACTAACG1800.0100.05922
Produced by FastQC (version 0.11.5)