FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904591
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTG3500.0119.332941
CGATTGA1600.0117.018291
CCGTTCA2550.0112.958831
ACTTTAG1500.0110.380643
TAAACCG2100.0109.731431
TACCTTA3050.0108.607141
TTGGCAA3900.0107.093651
TAGTTGG1550.0106.855411
ACGCCGA3800.0106.121851
GTATTGT1700.0105.863782
ATCCGAA2600.0105.2472151
TCAGCGA2150.0103.7957468
TGACTAG1750.0102.873221
CGTAACA1900.0102.2978443
AGTATTG1550.0102.175628
TACGGAT2550.0101.662951
AGCTAGT3050.0101.490398
TTGAACG3450.0100.189571
TATTGTC2600.099.674813
GTGCATA3950.098.445761
Produced by FastQC (version 0.11.5)