FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692124
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA700.0133.713569
CGATTGA1650.0122.278321
TTCGTAA1200.0119.9993445
AGCGTAT2150.0110.598911
GTCGTAA1900.0109.981531
CTTTGCG2500.0109.5264361
GTATTAG1250.0109.439398
TACACGA2650.0108.7650761
TACCTAT2800.0108.08531
AGATAGT1000.0107.9994058
TAAACCG1750.0107.055911
TTTGCGA950.0106.104682
GTGCATA3000.0105.68341
TATTAGC1300.0105.230199
ACTTTAG1300.0105.230193
CTAATGG2200.0104.809981
ACGCCGA2000.0104.482451
TAGTTGG1800.0104.082141
CCTTACG2150.0103.895941
CGGACGT1250.0103.761891
Produced by FastQC (version 0.11.5)