FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731015
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA2250.0134.46051
TGACTAG1650.0126.602421
GTTATGC1000.0122.3629768
ACTTTAG950.0121.292843
GGTATTG2300.0119.010071
CCTTACG2300.0115.878231
TTGAACG1800.0112.050421
AGCGAAC1350.0111.966119
CTTTGCG2400.0111.0499651
CCGTACA650.0110.735719
TAAACCG1650.0109.1400151
TACCTTA2250.0108.8489761
GATTGAC800.0108.0264362
GAATAAG3700.0107.075211
TTATGCA750.0105.625852
CTGATAG3900.0105.2781451
CGTCTAG1450.0104.244317
ACGCCGA2500.0103.726671
TAGTTGG1600.0103.546591
TACGAAA1600.0103.468699
Produced by FastQC (version 0.11.5)