FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467288
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3700.0112.901851
AGCGATA3650.0108.4880148
CGTTAGA4600.0108.0353851
CCTTACG4750.0107.656311
ATAACGC4650.0106.873721
CTCTACG4650.0103.775931
TGACTAG4800.0103.533911
CTAATGG4200.0102.890841
CGCTATC5400.0102.700311
TAAACCG4500.0100.833031
CGAACAT5700.099.8221741
TTGAACG4750.097.042311
TCGACTG2450.096.9759
GTCGACT3800.096.627018
TACGGTA4950.096.0314561
TCGTTAC3950.094.780328
TGTAGCG3500.094.624097
CGTCTAG3450.093.908597
ATGCGAT4000.093.630661
CCGTTCA4950.093.1214141
Produced by FastQC (version 0.11.5)