FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257551
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3050.0120.516891
CCTTACG3800.0106.214071
GATCTAG4350.0101.069051
CTAATGG4650.0100.748371
TTGAACG3650.0100.70591
CTCTACG4500.099.301721
TGCGTGG4550.098.21051
ATAACGC3750.096.098441
GTCGTAA2700.096.0984341
TACGGTA4000.093.6959841
CGCTTCA3700.093.501191
GGCTCGA3550.093.391441
AGCGATA2700.093.332468
ACGAGGA3750.092.254511
AACGTTG4300.092.187461
GACTAAC6500.092.032741
ACTAACG1800.092.00282
TATGCCC4000.091.894141
GCATGCG4000.091.894141
AACCGCG4100.091.410711
Produced by FastQC (version 0.11.5)