FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288496
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3100.0116.255831
CGCTATC4400.0113.032371
TCGTTAC3350.0109.6038748
TAAACCG4250.0106.8459551
GTCGACT2050.0101.846178
AGCGATA2900.0101.7856148
TGACTAG4000.0100.910061
TCGTAGG3000.0100.792598
TTACGGA1800.099.9926459
CGTTAGA4600.098.716361
TGTTACG2050.098.334237
ATAACGC3600.098.1071
ACGATGT4800.097.606461
ACGATAC3300.096.104831
CTCGAAA4500.096.104821
AGCGTAT3800.094.840291
TGCCCTA5700.094.840291
CCTTCGA4600.094.015591
TCCACGA3300.091.629628
CTAATGG4650.091.454591
Produced by FastQC (version 0.11.5)