FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695689
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC510953.013229430632622No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC299571.7666565036395236No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC152510.899398415629281No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC108930.6423937408333721No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC85610.5048685224708069No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC62540.3688176310632433No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT54130.3192212722969837No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT32320.1906009887426291No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22020.1298587181965561No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG37550.098.733491
GTCTCTC43550.088.7747962
GCTCAAA159950.082.848287
GGCTCAA159700.082.7075356
CTGATGG159800.082.375381
CTCAAAC161750.081.970828
TCTCTCA48050.080.439473
TGGCTCA165800.079.2715455
TCAAACA167200.079.2151649
ATGGCTC169350.077.737334
GATGGCT182600.072.173193
AGCTGGT56350.068.597319
GATACGG755.142283E-967.179684
AGTCTCT58750.066.6658251
TGATGGC205550.064.096932
TCAGCTG60750.063.3901027
CTCTCAG63700.060.7916534
CAGCTGG68550.056.912258
CTCAGCT68750.056.3278966
ATACGGC902.1709639E-855.9830635
Produced by FastQC (version 0.11.5)