FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422405
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG4650.0111.543631
CGATTGA3450.0110.6681
CGTTAGA4950.0104.783421
AGCGTAT4950.0101.8727651
TCCCGAT4350.0101.019641
GCATGCG4100.0100.151231
TGACTAG3650.098.6830141
ATAACGC4600.098.661571
TTGAACG4550.096.5792161
GACCAAT6150.096.0514761
GGCTCGA4900.095.561421
TAAACCG4750.095.545931
CTCTACG4400.094.959981
CGATCGG6250.094.514651
TCGTTAC4050.094.219728
CTTACCG5300.093.78611
GCGCGAT4250.093.226421
CTAATGG4250.093.226421
CGCTATC4650.092.9530261
AACCGCG3850.091.685491
Produced by FastQC (version 0.11.5)