FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700858
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC519843.0563398002655133No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC304411.7897437646176222No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC154050.9057193487051829No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC108500.6379133355047863No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC88990.5232065228255386No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC65650.385981663372251No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT55280.32501243490050313No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT33250.1954895705579184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19890.11694097919990969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG38200.0100.1340561
GTCTCTC45850.087.43462
GCTCAAA159150.082.172887
CTGATGG161450.081.963741
GGCTCAA160150.081.794616
TCTCTCA49500.080.9897843
CTCAAAC163200.080.0084158
TGGCTCA166600.078.541495
TCAAACA168800.077.612249
ATGGCTC170000.077.0134
CTTCCGA2700.074.640494
TCCGATC2700.074.640496
TTCCGAT2800.071.974765
GATGGCT183100.071.738913
AGTCTCT57900.068.801391
AGCTGGT59800.066.566439
TCAGCTG61500.064.601747
CCGATCT3050.063.7153667
TGATGGC208050.063.2722782
CCTAACG350.003669882761.6980938
Produced by FastQC (version 0.11.5)