FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743460
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG5050.0114.164221
CGTTAGA6150.0110.149921
TGACTAG5850.0109.639131
CGATTGA4350.0104.371691
TCGTAGG5000.0103.6740958
AGCGTAT4850.0102.527131
TAAACCG6150.0101.9472661
TCCCGAT5400.098.757341
CTCTACG6450.098.322831
AACCGCG4000.097.289321
TTGAACG6150.097.260031
ATGCGAT4850.096.583531
AGCGATA4550.096.521988
TCGTTAC5150.096.460528
CTTACCG6250.095.703881
CATCGAA6500.094.240371
CTTTGCG6200.092.98861
CCTTCGA7350.091.185771
ACGATGT5800.090.703971
GTCCGAA5800.090.615538
Produced by FastQC (version 0.11.5)