FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747393
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3750.0111.376131
CGCTATC5750.0110.207271
CGGCTAG5600.0105.443851
TAAACCG6350.0104.330071
CGTTAGA5800.0104.290971
TGCCCTA6700.099.954781
TCGCGAG6200.098.723981
TATGCCC7000.097.728441
CGAACAT8850.097.641261
TGACTAG5200.096.937121
GTCGTAA4100.096.5993651
TTGAACG5150.095.081741
GCATGCG5000.095.0537641
TCCCGAT6750.093.880271
CTAATGG5600.092.584851
GACGCGT6700.091.356521
CCTTACG5450.091.169171
ACGATGT5800.090.633821
ATTACGT4450.090.622463
GACCAAT8350.090.552041
Produced by FastQC (version 0.11.5)