FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422405
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC431093.030712068644303No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC248831.7493611172626642No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC131470.9242796531227041No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC88400.6214826297714083No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC73800.5188395710082571No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC53110.37338170211718885No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT46020.3235365454986449No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT27460.19305331463261166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17350.12197651161237483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG33600.0101.746061
GTCTCTC35350.083.291822
GGCTCAA133500.079.896646
GCTCAAA134450.079.33217
CTCAAAC136400.078.46738
CTGATGG137300.078.217281
TCTCTCA37850.077.958643
TGGCTCA139750.076.271945
TCAAACA141100.076.006619
ATGGCTC143800.074.524214
GATGGCT154550.069.384683
AGTCTCT45950.064.5111541
AGCTGGT47750.061.952749
TGATGGC173800.061.799862
TCAGCTG49800.059.253787
CTCTCAG51250.057.577344
CTCAGCT55300.053.750996
CAGCTGG57550.051.903298
TCTCAGC57000.051.895365
TCCGATC1358.43722E-426.6561746
Produced by FastQC (version 0.11.5)