FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598671
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA5500.0110.058321
CGATTGA4050.0106.7585141
GGCTCGA4650.0103.831271
TACGGAT4350.0102.7090451
CCGGTAG3650.0102.663671
CGTTAGA4650.0102.281551
CGCTATC5650.0102.034681
TGACTAG5750.0101.513421
CCTTACG5100.098.908621
CGGACGT4300.098.875761
TAAACCG5500.098.266361
TCCCGAT5500.096.956141
GTCGACT2750.096.871248
GTCGTAA3500.096.768971
TCGGATG4250.096.5632258
GTCGAAA1300.094.15248
TAGTTGG4750.094.0598751
CGAACAT7550.093.537441
CATCGAA6550.093.515571
GCATGCG5650.093.106651
Produced by FastQC (version 0.11.5)