FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598671
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC479502.999366348673367No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC275591.7238693890112475No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC147160.9205146024416531No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC101670.6359657490503049No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC82150.5138643285579084No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC58670.3669923330066036No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT51480.32201747576580797No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT30440.19040815777605274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17990.11253097103781828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG33650.097.195351
GTCTCTC39100.089.453922
GGCTCAA146300.081.811616
TCTCTCA42500.081.7921453
GCTCAAA146350.081.783667
CTGATGG146700.081.566951
CTCAAAC149150.080.108578
TGGCTCA153000.077.75865
TCAAACA154950.077.298229
ATGGCTC157100.076.001764
GATGGCT167450.071.390083
AGCTGGT51350.067.563849
AGTCTCT52950.066.666071
ATACGGC1100.065.42965
TGATGGC187250.063.8776632
TCAGCTG54650.063.4780927
CTCTCAG54700.063.4180874
TACGGCG954.8748916E-1060.6084756
TCCGATC2050.059.6845666
TGATACG1102.7284841E-1158.8848083
Produced by FastQC (version 0.11.5)