FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639299
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA4400.0108.0804141
CGTTAGA5600.0105.5070651
CGCTATC6000.098.473261
CCTTACG5500.098.254911
GACCAAT6900.095.027221
GTCGTAA3000.093.6696851
ACGATAC4950.093.160221
GTCGAAA1800.091.9926768
TAAACCG5750.091.476751
TCGCGAG6150.091.3850561
CCCAATC7650.091.362091
CGATCGG4900.091.169871
TTAGGCA5150.090.9414441
CCGGTAG3650.090.807281
CGTCTAG3100.090.573437
TGCGTGG5650.090.5452351
GACGCGT5750.090.223651
ATGCGAT5200.090.0671
AGCGATA3850.089.759088
GCATGCG4500.089.666711
Produced by FastQC (version 0.11.5)