FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636255
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA4200.0118.313891
CCTTACG5150.0113.2696841
CGTTAGA5700.0108.6574551
TAGTTGG4300.0105.5135041
ACGATAC4550.0104.464431
TCCCGAT5600.0104.167661
ATAACGC5500.0103.442811
TGACTAG4450.0101.9568561
TGCGTGG5550.0101.213291
AGCGATA3700.0101.1854558
GATCGCG5350.0100.958621
GATCTAG5350.0100.958621
TAGCCGG6500.098.60811
CTCTACG5650.098.147271
TACGGAT3600.096.022861
CGCTATC5400.094.689211
AGCGTAT5050.094.121421
TAAACCG6000.093.622291
TTAGGCA5550.093.427651
GCATGCG4650.092.9253541
Produced by FastQC (version 0.11.5)