FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430794
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3050.0120.480931
CGTTAGA5250.0111.1664351
CGCTATC4750.0109.216151
AGCGATA3450.0108.520898
TAAACCG5200.0108.0784761
GCATGCG4550.0107.682591
TCCCGAT5250.0104.3043061
TGACTAG4700.0104.245911
GCGCAAC4450.0102.006651
CCCAATC5500.0100.8732451
CCTTACG5000.099.43221
GATCTAG4800.097.570851
CATGCGA1800.095.999262
CGTCTAG2200.094.908357
CCTTCGA6000.094.868891
TCGCGAA1750.094.627839
GATCGCG5050.094.167381
TACCTTA4400.093.34051
GTCTAGT3250.093.0454258
CGAACAT5900.092.8131561
Produced by FastQC (version 0.11.5)