FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024852
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT3850.0119.68131
GCGCGAT3300.0109.084511
TGACTAG4450.0108.398131
CCTTACG3400.0107.993671
TACACGA3750.0105.593821
TAGTTGG3000.0105.593811
GGTATTG5200.0105.22461
TATTGTC2200.0104.716023
ACTTTAG2350.0104.159033
ACCGAAT1750.0102.831049
TACCTTA4150.0102.3554461
CCTACCA3450.0102.254881
CGATTGA2750.0102.10311
CTGCGTA1700.0101.621279
TAAACCG4500.0100.794091
CTTGACG1500.0100.774429
GTCGACT2300.0100.14858
ACTAGTC2250.099.189353
TACGCAT1550.097.537914
GGTGCGT1850.097.2915951
Produced by FastQC (version 0.11.5)