FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830240
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3050.0127.569021
CCTTACG3400.0118.6753161
GGTATTG3950.0116.743881
TACGGTA2700.0112.0822451
TAGGCTA1500.0110.367957
TAAACCG3400.0110.198511
CGAACAT2950.0107.468691
GTATTGT1150.0106.480842
GTCGTAA2100.0106.363761
CACCGAA2250.0105.569348
TACGGAT3050.0103.945131
GCGCGAT2300.0103.380211
GATTGAC1550.0102.237782
TACCTTA2900.0101.867861
TATGCCC2700.0101.4077451
TCCCGAT2800.0100.359361
AGCGTAT1800.0100.073431
TAGACTA1800.099.970977
GTATTGC1300.099.735462
TTGCCGT3400.099.602491
Produced by FastQC (version 0.11.5)