FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805306
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG2550.0118.603631
TAGTACG1350.0117.312137
CCTTACG3600.0116.015051
AGCGATA1700.0114.3322758
CGATTGA2550.0112.955831
TTACGGA1100.0111.2526259
GGTATTG3350.0107.476641
TCTAACT3350.0105.29448
GACCAAT4050.0104.9025041
TAGACTA1650.0104.708357
CTAATGG2350.0101.119511
TATTACC2850.0101.065761
CGAACAT3000.0100.813081
GGACGAA1000.0100.7817849
GCCGTAG1650.0100.357973
TAAACCG3550.099.393181
GTCGTTG1450.099.2924048
TCGTTAC2250.099.182088
TGTAGCG1850.097.279737
TACCTTA3850.097.259381
Produced by FastQC (version 0.11.5)