FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348502
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA4350.0124.1966551
CCTTACG4900.0113.1963651
TAAACCG5550.0111.620341
TACGGAT3800.0109.946711
TGACTAG4800.0109.551791
AGCGATA3500.0104.9094248
GGTATTG6050.0104.776811
TACCTTA5250.0104.277871
CCGATAG2500.0100.828973
CGGACGT4300.0100.5126341
TGGTTAG3550.099.427291
GCCGTAG3200.099.028463
CTTTGCG3450.098.1333541
TTACGGA2000.097.195499
GTATTGT2300.097.071372
TCTAACT6050.096.392228
GACCAAT6450.096.045411
TAGCCGG6300.094.902011
TTGCCGT4950.093.134941
ACGATAC3650.092.756191
Produced by FastQC (version 0.11.5)