FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314975
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA4200.0118.361761
GGTATTG6150.0113.633991
CCTTACG5000.0110.9512941
TATTGAG2550.0110.108843
TACGGAT3700.0109.043031
GTCGTAA2750.0107.414471
TTACGGA1700.0105.8738869
AGCGTGA3500.0102.848918
TACACGA5000.0102.305741
CTCTACG3950.0102.141581
GACCAAT6450.099.412711
TATGCCC3850.099.1805951
GTATTGA1900.098.518432
ACGCCGA4650.097.61111
CTGATAG4800.097.5626751
AGCGTAT3250.097.53961
TAAACCG4950.097.5172041
TGACTAG4600.097.105871
CATATTC5350.096.9594961
TAATAGC2850.095.992329
Produced by FastQC (version 0.11.5)