FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391600
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA3850.0127.2683941
CCTTACG5550.0115.5498661
GCCGTAG3300.0113.527043
TAAACCG5100.0111.6167451
GTCGTAA3850.0110.424051
TGACTAG5150.0109.133931
TAGTACG2600.0107.975827
GCGCGAT3300.0106.99281
CTCTACG4250.0106.812981
GGTATTG5900.0105.031331
TACCTTA5250.0104.310191
TCTAACT7350.0103.813498
TACGGAT4150.0102.44161
ATCTAAC2050.0101.8308647
TATGCCC3900.0101.617971
GACCAAT7700.0101.066081
TAGCCGG6150.099.590121
CTCGTAC5250.098.8201751
TTACGGA1750.098.7207649
TCCCGAT4600.098.685911
Produced by FastQC (version 0.11.5)