FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343127
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA4150.0117.996231
CCTTACG4900.0117.571271
TAAACCG4950.0116.3836751
CTAATGG3750.0111.379171
TACGGAT3950.0111.209021
TCCCGAT4300.0107.1812441
TAGCCGG6400.0106.893411
CTTTGCG2900.0106.777011
TTTGCGA1150.0106.421412
TACCTTA5800.0105.5354161
TGGTTAG3600.0104.0179141
GGTATTG6000.0103.217771
TCTAACT5950.0102.840398
CCTTCGA5750.0100.191161
CACGCGA1950.099.676078
ACGCCGA5150.097.880931
ACGAGGA4050.097.794621
GACCAAT6650.096.3774951
GTCGTAA4200.096.016531
CACCGAA3450.095.984378
Produced by FastQC (version 0.11.5)