FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917918
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG3700.0116.7952041
TACGGAT2900.0114.244491
CGATTGA3050.0110.987351
GGTATTG3350.0107.498071
CTCTACG2950.0107.4251861
TGTAGCG2300.0106.429237
TACACGA3800.0106.140191
TACCTTA4300.0105.523111
GTCGTAA2050.0105.4005361
CTAATGG2100.0102.8911
AGTATTG1800.099.994788
CCTTCGA3250.099.725131
TAGCCGG4350.099.343051
TGACTAG3050.099.180181
GCGCGAT2400.099.032591
TAAACCG3450.098.119251
GGCTCGA2500.097.952241
AGCGTGA3050.096.781848
ATTACGA1200.095.994999
GTTAACG900.095.994999
Produced by FastQC (version 0.11.5)