FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894542
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT2600.0116.458411
TAAACCG3600.0116.150331
CGATTGA2700.0114.815271
GCCGTAG2300.0112.690323
CCTTACG3300.0111.416921
CCGTAGC1250.0109.434824
GCGCGAT2450.0105.933021
CTTAACT4100.0103.744031
TAGTTGG2400.0102.132181
CTGCGTA1200.0101.995179
CCTTCGA2850.0101.183591
GACCAAT4350.0101.096361
TAGACTA1500.0100.795227
CTAATGG2300.0100.3037341
TGACTAG2600.099.8214951
CGTTGGG2100.099.557421
ACCGAAT1600.098.9953169
CTCTACG2800.097.840921
CGAACAT2950.097.753641
TACTCGC3100.097.6748051
Produced by FastQC (version 0.11.5)