FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944286
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTG4150.0125.010651
GCGCGAT2350.0116.514081
CCTTACG4100.0115.990581
TGACTAG3750.0111.444681
CGATTGA3050.0111.0351941
TACCTTA3500.0107.052771
CTTTGCG2300.0103.38291
TTCGGAA1400.0102.837257
TAGCCGG4650.0102.271261
TACGGAT2900.0101.8705141
CATGCGA850.0101.70282
TAAACCG3050.0101.585391
CTAATGG2500.0100.876651
TTTAGCG502.055458E-10100.849935
GACCAAT4700.099.65021
ACGAGCC2750.099.566571
CTGATAG3500.098.817951
CCTACCA3150.098.360461
TTTGCGC3100.097.622571
GTATTGT1550.097.6018752
Produced by FastQC (version 0.11.5)