FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914174
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG3500.0125.501641
TGACTAG3600.0116.0147251
CGATTGA3800.0115.593621
CCTACCA3550.0111.563451
GAAGCGT1250.0109.429956
GACCAAT5350.0109.023181
GGTATTG3050.0108.603941
CGAACAT3300.0104.740561
GGCTCGA2750.0104.740561
ACGATAC3200.0103.513131
TACCTTA3300.0102.558471
TAGCCGG4150.0102.3744351
CATATTC3450.0102.273841
AGCGTAT2550.0101.6599661
GCGCGAT2550.0101.6599661
CTCTACG3350.0101.027741
GCCTCTA3800.0100.43381
TAAACCG3450.0100.186621
TACGCAT1150.0100.164714
CGAATGT4250.096.5769651
Produced by FastQC (version 0.11.5)