FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579577
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA1300.0121.8165058
GACGACG600.0119.970819
CCTTCGA1800.0115.981791
TTGAACG1600.0112.4823461
CCTTACG2050.0112.3726041
CGATTGA2000.0111.582481
CGTTAGA1950.0110.751851
TAGTTGG1850.0108.955871
GGTATTG1850.0108.955871
GACGGCT2150.0107.145971
ATAACGC2150.0107.145971
ACGATGT1750.0106.9546361
CAACGAT1350.0106.640728
GTCGTTA900.0103.97477
AACGTTG2450.0102.8409961
TTAGGAC1500.0100.784171
TACCTTA2000.0100.784171
CTTACCG3000.0100.784171
ACTAGTC1000.0100.775483
ACTACGT502.0736479E-10100.775483
Produced by FastQC (version 0.11.5)