FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466615
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA1400.0128.636341
GTCGTAA1250.0126.7839661
GGTATTG1750.0115.258151
CACCGAA1300.0110.765778
TACCTTA2150.0110.567421
CTGATAG1500.0110.4557341
CCTTACG1400.0108.054521
CGTCTAC1400.0108.054521
TCGTAAC600.0107.996622
CTCGAAA2150.0107.2168961
TCAACGA1750.0107.025421
AGCGATA1500.0105.59678
TACACGA1050.0102.909071
AACTGCG1000.0100.7968449
CCTACCA2450.099.968811
TACTTAC2100.099.478771
TACGGAT1450.099.360481
CGTAAAT950.098.523237
GACCAAT2050.098.3911061
ACGAAGC1100.098.178769
Produced by FastQC (version 0.11.5)