FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476219
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA1500.0115.204121
TTTAGAC750.0115.1799245
AGCGATA1350.0111.980488
CCTTACG1750.0111.089681
GTCGTAA1300.0110.773191
CGTATAG650.0110.749933
TACGGAT1700.0110.121581
CGATTGA1900.0109.898661
CCCGATA600.0107.981182
CGTTAGA1150.0106.438581
CCGTTCA1300.0105.2345351
TTAGGAC2100.0102.860821
GCGCGAT1050.0102.860821
GGCTCGA1050.0102.860821
CTAATGG1850.0101.19281
TACCTTA2000.0100.80361
TTGCGAG502.055458E-10100.782433
TCCGCAA1150.0100.1564568
TCCGACG650.099.6749349
ATTACGT1300.099.6749343
Produced by FastQC (version 0.11.5)