FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722980
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG2400.0123.037831
CACCGAA1700.0122.81888
TTACGGA600.0119.995399
GTCGTAA2300.0112.730311
CGTAATC1500.0105.625173
ACGATAC2600.0105.263131
CCTTCGA2150.0103.845881
CTCTACG2650.0103.277031
GGTATTG2800.0102.888781
TGACTAG2100.0102.888771
ATTACGT1150.0100.197783
CTGATAG2950.0100.0985641
CGGACGT2450.099.949091
TGTTCCG3100.099.901681
CGTAACA2550.098.847083
TTGCGTC2200.098.212011
CGATTGA1550.097.5783841
CTTACCG3050.096.816641
ATAACGC3500.096.715451
GACGACG1200.095.9963159
Produced by FastQC (version 0.11.5)