FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705145
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA2700.0117.4226761
CCTTACG2200.0114.6326751
TGGTTAG1700.0114.440011
GTGCATA3650.0112.5239641
CTCTACG2200.0111.357461
ATAACGC3000.0110.484061
GTCGACT1650.0109.088818
TTAACTC1950.0107.158451
ATTACGA1350.0106.664639
CGTAACA2100.0106.2836763
GGTATTG2250.0105.6804051
TTTGCGA750.0105.597972
TGACTAG2800.0105.508841
TCGTAAC1300.0105.2287452
CGATTGA2750.0104.8070141
CCGGTAG1950.0103.4633261
CCTACCA2950.0102.586531
CGTTAGA1850.0101.2662351
CTTTGCG1850.0101.2662351
GATCGTA3650.0100.679341
Produced by FastQC (version 0.11.5)