FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581716
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGA450.0127.998639
GATTACG351.9917934E-9123.459082
CGTACGT301.1464181E-7120.019343
GTCGTAA1800.0116.068591
TCGTTAC1250.0115.198768
CCTACCA2500.0112.386421
GTACGAT1000.0107.998859
CGTTAGA1500.0105.662441
CCTTACG1450.0104.337521
CGTCGAA700.0102.856047
CGTAACA1850.0101.205493
CCTTCGA1650.0100.422971
TACGGAT1300.099.751251
CGGACGT2750.099.549741
ATAACGC3050.099.206161
CTTACCG2550.098.881961
AGTACGA2050.098.340418
GCATAGT1400.097.7300343
CATGGCG2600.096.9803851
TAGTTGG1500.096.056761
Produced by FastQC (version 0.11.5)