FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745224
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA2400.0114.1002961
CCGATAG2000.0111.653183
CGTCTAC1950.0110.866691
CCCGATA1650.0109.14292
AGCGAAC1350.0106.631589
GACGACG1150.0106.399779
AGCGATA1850.0105.046528
CGATTGA2350.0104.261861
ATAACGC3750.0103.771221
GGCCGAT1950.0103.475581
ACGAGGA2650.0103.336131
CACGCGA1400.0102.82338
CCTTACG2250.0102.49011
TTGAACG1900.0102.405811
CTCTACG2350.0101.1953351
AGTACGA3000.0100.766848
CGTAACA2650.0100.576213
GTACGAT1650.0100.330629
TCGTTAC2450.099.88558
CCTTCGA2750.099.5784451
Produced by FastQC (version 0.11.5)