FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717850
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC2200.0117.884341
ATCCGAA3150.0109.7758941
GGTATTG2900.0109.302731
CCTTCGA2350.0107.2942661
ATTACGT1750.0107.016583
CTGATAG3100.0106.8987051
CGTTAGA2250.0102.4575041
CGTAACA2200.0101.4973753
CGATTGA1850.0101.246011
CTTAACT2350.0101.163161
AACGATG1850.0101.161399
TTGAACG2500.0100.8566061
TAGTACG1500.0100.772317
TAGTTGG2650.0100.5847551
GTCGTAA2150.0100.521541
CTCTACG2300.0100.230161
CGGACGT2950.0100.1239851
GTACGAT1600.098.97289
TGAGCCG2850.098.581641
TACGGAT2950.097.681941
Produced by FastQC (version 0.11.5)