FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694142
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA1350.0128.006151
CCTTACG1950.0114.467041
TTGAACG2150.0113.865941
GGTATTG2000.0111.605361
CGTTATG650.0110.750627
TACCTTA2150.0110.516941
TTACGGA850.0110.099149
TAAACCG2150.0107.167941
TACGGAT2200.0104.73231
TGGTTAG2200.0104.73231
ATAACGC3100.0104.521151
TAGTTGG2550.0104.47561
TGACTAG2200.0101.459411
GTGTCGA502.055458E-10100.790316
TTCGGTA800.098.9904864
CTTTGCG1750.098.74761
CATATAC2050.098.3249368
CTCTACG2500.097.9247061
TTGCGTC2950.097.6318051
CCTTCGA1850.097.301971
Produced by FastQC (version 0.11.5)