FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567217
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG351.9954314E-9123.428047
GTACGAA900.0111.999528
CTACCCA1550.0106.904221
AACGTTG1900.0106.170321
TGAGCCG1900.0106.170321
TACGAAA950.0106.1048059
GCATGCG1700.0105.947271
CGGACGT1700.0105.947271
CGTTAGA1650.0104.791481
ACGAGGA1650.0104.791481
GGTATTG2550.0104.5346451
GACGACG900.0103.999569
CTTTGCG1700.0101.709381
GATCGTA2500.0100.861811
GTACGTA2000.0100.861811
CACCGAA1950.099.691888
GTCGACT950.098.5258948
TAGTTGG2200.098.242011
CGCTATC2500.097.980041
AGCGAAC1250.097.919589
Produced by FastQC (version 0.11.5)