FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599578
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG450.0127.9822857
GTCGTAA2150.0123.972541
TTACGGA600.0119.983399
TACGGAT1350.0117.395421
CGGACGT2250.0112.059261
CCTTCGA1500.0110.458411
ACGAGGA2350.0107.290781
CCTTACG1950.0107.1335751
ACGATGT1350.0106.723111
CGTACGG950.0106.090589
GGTATTG2250.0105.655871
TCGTTAC1500.0105.585388
ACGAGCC1850.0105.136671
TACCTTA2200.0104.7826841
TACGTTA553.6379788E-12104.773945
CCGTTCA1450.0104.331041
TCGTAAC1250.0103.7348562
TGAGCCG1950.0103.4393161
CGTCTAC1750.0102.911561
CTAATGG2100.0102.911561
Produced by FastQC (version 0.11.5)