FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185337
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA500.0143.988131
CTTCGAC100.0072361557143.988132
TTCTAGT253.857349E-8143.988133
CGTATGA100.0072361557143.988137
CTATCGT100.0072361557143.988135
TGGACGA151.2631506E-4143.988139
AACTTCG100.0072361557143.988134
CGATTCG253.857349E-8143.988134
CGCGAGA151.2631506E-4143.988132
AACGGAC253.857349E-8143.988132
CGTAGAA151.2631506E-4143.988135
ACTCGAA151.2631506E-4143.988136
TATCGCG151.2631506E-4143.988136
CCGTTAC100.0072361557143.988136
ATCGACG100.0072361557143.988139
TCTATCG253.857349E-8143.988138
CCCGATA450.0127.989442
TGCGGGT403.45608E-11125.989621
CTTTGCG800.0125.989621
CGTTGGG750.0124.789711
Produced by FastQC (version 0.11.5)