FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196353
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTC151.2649687E-4143.939394
GTACCGA202.2054282E-6143.939398
ATAGACG100.00724381143.939396
TAGTTGT100.00724381143.939396
TGCGAAG151.2649687E-4143.939393
CGTACGT100.00724381143.939393
CGTACGA151.2649687E-4143.939397
GACGCAC151.2649687E-4143.939399
TAGGATG100.00724381143.939397
GACCTTA100.00724381143.939392
GCGTAAT151.2649687E-4143.939399
AGTCGTC100.00724381143.939396
TACGGAT550.0130.920671
TAGTTGG500.0129.611471
GCGCCAA750.0124.8110351
CTCGAAA750.0124.8110351
CATCGAA650.0121.856931
CTAATGG600.0120.010621
AGCGTAT900.0120.010611
CGCGATA301.14754585E-7119.949492
Produced by FastQC (version 0.11.5)