FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211359
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAC202.2034383E-6143.970679
CTGTATA202.2034383E-6143.970676
GTATTAG100.007239495143.970678
GAATGGT450.0143.970673
ATAGCGC100.007239495143.970676
CCGTAGC100.007239495143.970676
TCGTGAC202.2034383E-6143.970678
ATATCGA151.2640106E-4143.970665
GGCTCGA600.0143.970661
TTTATGC650.0132.8961
GCGCCAA550.0130.882431
GACACGC450.0127.973931
TCGCATA403.45608E-11125.9743359
TCGCGAT403.45608E-11125.9743358
GCGATAT351.9917934E-9123.4034359
GTACGCG351.9917934E-9123.4034359
AGCGTAT900.0119.9755551
CTGATAG900.0119.9755551
CGTGATA301.1463635E-7119.975557
CGCTAGC301.1463635E-7119.975559
Produced by FastQC (version 0.11.5)